2.7.8.27	H285A	site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology	691074	
2.7.8.27	H328A	site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology	691074	
2.7.8.27	H332A	site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology	691074	
2.7.8.27	additional information	construction of an SMS1 interference vector, enzyme silencing by shRNA in Hep-G2 cells leading a downregulation rate of 45% and reduced enzyme activity of 56%	738307	
2.7.8.27	additional information	construction of an SMS2 interference vector, enzyme silencing by shRNA in Hep-G2 cells leading to a downregulation rate of 67% and reduced enzyme activity of 63%	738307	
2.7.8.27	additional information	downregulation of SMS in Huh7 cells by expression of siRNA, overview	691046	
2.7.8.27	additional information	downregulation of SMS1 or SMS2 by siRNA oligonucleotides targeting SMS1 or SMS2 in HeLA cells reduces de novo synthesis of sphingomyelin, overview	690832	
2.7.8.27	additional information	endogenous SMS2 expression in HeLa cells is reduced by about 80% after treatment with SMS2 siRNA, but is unaffected after treatment with lamin A or SMS1 siRNAs. Impact of SMS1 and SMS2 depletion on the lipid composition of HeLa cells, overview	692991	
2.7.8.27	additional information	generation of SMS2 KO mice	-, 737776	
2.7.8.27	additional information	knockout of SMS1 in T-cells by transfection of SMS1-short-interfering RNA into Jurkat T cells, impaired TCR signaling through dysfunction of lipid rafts in SMS1-knockdown T cells, overview. CD3-induced tyrosine phosphorylation and association of linker for activation of T cell with ZAP-70 and Grb2 and phosphorylation of protein kinase C are also severely impaired in Jurkat-SMS1/kd cells	692624	
2.7.8.27	additional information	overexpression of SMS1 in Jurkat cells leads to suppression of the production of dihydroceramides and dihydrosphingosine, and ceramide	690695	
2.7.8.27	additional information	overexpression of SMS1 is accompanied by attenuated ceramide response and apoptotic resistance after photodamage with the photosensitizer Pc 4, while suppression of SMS1 by small interference RNA is associated with enhanced ceramide production and apoptosis after photodamage, overview	692213	
2.7.8.27	additional information	S49AR cells show a defect in the synthesis of sphingomyelin and downregulated SMS1, phenotype, overview. Resistance to ALP can be mimicked by small interfering RNA-induced SMS1 suppression, resulting in sphingomyelin deficiency and blockage of raft-dependent internalization of ALP and induction of apoptosis, overview	690804	
2.7.8.27	additional information	sphingomyelin synthase 2 deficiency, due to siRNA treatment of HEK-293 cells, attenuates NFkappaB activation, it impairs TNF receptor 1 recruitment tolipid rafts and TLR4-MD2 complex recruitment to plasma membrane	690656	
2.7.8.27	additional information	sphingomyelin synthase 2 knockout deficiency attenuates NFkappaB activation, it impairs TNF receptor 1 recruitment tolipid rafts and TLR4-MD2 complex recruitment to plasma membrane	690656	
2.7.8.27	S273A	site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology	691074	
2.7.8.27	S283A	site-directed mutagenesis, inactive mutant with wild-type localization pattern, overview. The point mutation only influences SMS2 catalytic activity but not the enzyme topology	691074