2.1.1.140	E204A	4-6% of wild-type activity	755791	
2.1.1.140	E204G	mutation to the corresponding residue of Papaver somniferum reticuline N-methyltransferase. Mutant shows a weak activity with (S)-coclaurine and a more substantial activity with (S)-N-methylcoclaurine. Compared to wild-type, activity with (S)-reticuline is 35fold improved	757949	
2.1.1.140	E207A	60-75% of wild-type activity	755791	
2.1.1.140	F332A	0.6% of wild-typ activity with substrate coclaurine	755791	
2.1.1.140	G331A	negligible effect on activity	755791	
2.1.1.140	H208A	1% of wild-type activity with substrate heliamine, 4% with substrate coclaurine, respectively	755791	
2.1.1.140	additional information	virus-induced gene silencing in opium poppy to functionally characterize six genes potentially involved in papaverine biosynthesis, e.g. the gene encoding coclaurine N-methyltransferase, altered alkaloid content phenotypes, overview. Norlaudanine is only detected in CNMT-suppressed plants	720710	
2.1.1.140	R330A	negligible effect on activity	755791	
2.1.1.140	W329A	4% of wild-type activity with substrate heliamine, 0.5% with substrate coclaurine, respectively	755791	
2.1.1.140	Y328A	5% of wild-type activity with substrate heliamine, 10% with substrate coclaurine, respectively	755791