1.14.19.9 F103A the mutant shows increased Km and reduced kcat values compared to the wild type enzyme 726682 1.14.19.9 F103A/W455A inactive 726682 1.14.19.9 H101A the mutant shows increased Km and reduced kcat values compared to the wild type enzyme 726682 1.14.19.9 K79A inactive mutant with abolished FAD binding 685105 1.14.19.9 K79M inactive mutant with abolished FAD binding 685105 1.14.19.9 additional information for optimization of the enzymatic regioselective halogenation process, a method that is easily scalable up to gram scale is established. An active FAD-dependent halogenase is co-immobilized with all auxiliary enzymes necessary for halogenation, formation of cross-linked enzyme aggregates (CLEAs) might stabilize its quaternary structure 744027 1.14.19.9 additional information gene disruption mutant, no production of pyrroindomycin B, but still production of pyrroindomycin A -, 676785 1.14.19.9 W455A the mutant shows increased Km and reduced kcat values compared to the wild type enzyme 726682 1.14.19.9 Y455W the mutant preferentially uses tryptamine as substrate 727668