1.14.19.24 16del_E site-directed mutagenesis, the product E/Z isomer ratio is 33:67 745076 1.14.19.24 A161G_T163M site-directed mutagenesis, the product E/Z isomer ratio is 36:64 745076 1.14.19.24 A88S site-directed mutagenesis, the product E/Z isomer ratio is 32:68 745076 1.14.19.24 D258E site-directed mutagenesis, mutating the aspartic acid into glutamic acid transforms the Choristoneura parallela enzyme into a desaturase with Choristoneura rosaceana-like activity that produces a mixture of (11Z)-tetradecenoate and (11E)-tetradecenoate in a 64:36 E/Z product ratio 745076 1.14.19.24 E258D site-directed mutagenesis, mutating the glutamic acid into aspartic acid transforms the Choristoneura rosaceana enzyme into a desaturase with Choristoneura parallela-like activity that produces an almost pure (11E)-tetradecenoate product 745076 1.14.19.24 E258D site-directed mutagenesis, mutating the glutamic acid into aspartic acid transforms the Choristoneura rosaceana enzyme into a desaturase with Choristoneura parallela-like activity that produces an almost pure (11E)-tetradecenoate product, the product E/Z isomer ratio is 86:14 745076 1.14.19.24 F252L site-directed mutagenesis, the product E/Z isomer ratio is 33:67 745076 1.14.19.24 H116N_I118V site-directed mutagenesis, the product E/Z isomer ratio is 42:58 745076 1.14.19.24 I103M site-directed mutagenesis, the product E/Z isomer ratio is 36:64 745076 1.14.19.24 I174V site-directed mutagenesis, the product E/Z isomer ratio is 35:65 745076 1.14.19.24 I65V site-directed mutagenesis, the product E/Z isomer ratio is 35:65 745076 1.14.19.24 K286Q site-directed mutagenesis, the product E/Z isomer ratio is 35:65 745076 1.14.19.24 K309N site-directed mutagenesis, the product E/Z isomer ratio is 34:66 745076 1.14.19.24 L12M site-directed mutagenesis, the product E/Z isomer ratio is 31:69 745076 1.14.19.24 L19W site-directed mutagenesis, the product E/Z isomer ratio is 34:66 745076 1.14.19.24 L69I site-directed mutagenesis, the product E/Z isomer ratio is 30:70 745076 1.14.19.24 M250I_T251A site-directed mutagenesis, the product E/Z isomer ratio is 35:65 745076 1.14.19.24 additional information a second non-functional ezi-inserted desaturase gene exists in Ostrinia latipennis, in which an ezi-like element is integrated in intron 1 of gene latpg1. Only the functional latpg1 is expressed in the pheromone gland of Ostrinia latipennis 745075 1.14.19.24 additional information a second non-functional ezi-inserted desaturase gene exists in Ostrinia nubilalis. The non-functionality of ezi-inserted desaturase genes may not be a direct consequence of the insertion of an ezi- or ezi-like element into the gene 745075 1.14.19.24 N259S site-directed mutagenesis, the product E/Z isomer ratio is 31:69 745076 1.14.19.24 Q33E site-directed mutagenesis, inactive mutant 745076 1.14.19.24 S109A site-directed mutagenesis, inactive mutant 745076 1.14.19.24 T95A site-directed mutagenesis, the product E/Z isomer ratio is 35:65 745076 1.14.19.24 V321I site-directed mutagenesis, the product E/Z isomer ratio is 35:65 745076 1.14.19.24 W45G site-directed mutagenesis, the product E/Z isomer ratio is 31:69 745076