1.14.15.3 D323E gene CYP4A1 285379 1.14.15.3 E320A gene CYP4A1 285379 1.14.15.3 E320A/D323E gene CYP4A1 285379 1.14.15.3 H138A no activity 658713 1.14.15.3 H142A no activity 658713 1.14.15.3 H163A ~50% of activity 658713 1.14.15.3 H168A no activity 658713 1.14.15.3 H172A no activity 658713 1.14.15.3 H173A no activity 658713 1.14.15.3 H181A no activity 658713 1.14.15.3 H206Y gene CYP4A7 285384 1.14.15.3 H206Y/S255F gene CYP4A7 285384 1.14.15.3 H273A no activity -, 659069 1.14.15.3 H273Q no activity -, 659069 1.14.15.3 H308A ~30% of activity 658713 1.14.15.3 H312A no activity 658713 1.14.15.3 H315A no activity 658713 1.14.15.3 H316A no activity 658713 1.14.15.3 H318A ~25% of activity 658713 1.14.15.3 K18A complete loss of activity, mutant protein display an altered secondary structure and decreased melting temperature 744267 1.14.15.3 K193A 5-20% loss of activity 744267 1.14.15.3 K359A 5-20% loss of activity 744267 1.14.15.3 L69F oxidizes only C10 and C11 alkanes -, 659069 1.14.15.3 L69W oxidizes only C10 and C11 alkanes -, 659069 1.14.15.3 additional information development of a whole-cell biotransformation system expressing AlkB in Escherichia coli to regioselectively synthesize 2,2-, 3,3- and 4,4-difluorooctan-1-ols, from simple and inexpensive starting materials, method evaluation, overview -, 713529 1.14.15.3 additional information enzyme knockout mutant, no growth on alkanes of C12 to C16, but able to grow on octadecan and eicosan 661999 1.14.15.3 additional information in the insertion mutant lines cyp86b1-1 and cyp86b1-2 ralph1 (root aliphatic plant hydroxylase1) and ralph2, respectively, transcript levels are at the detection limit. Transgenic lines ralph3 and ralph4 expressing RNAi constructs show significant down-regulation of CYP86B1 transcript levels 706305 1.14.15.3 R90W gene CYP4A7 285384 1.14.15.3 R90W/W93S gene CYP4A7 285384 1.14.15.3 S255F gene CYP4A7 285384 1.14.15.3 W55C prefers C11-C13 alkanes as growth substrates -, 659069 1.14.15.3 W55S prefers C11-C13 alkanes as growth substrates -, 659069 1.14.15.3 W93S gene CYP4A7 285384