1.14.14.82 additional information an enzyme-deficient mutant line shows altered flower color, i.e. fuchsia flowers instead of bright blue due to accumulation of dihydrokaempherol instead of dihydroquercetin 660143 1.14.14.82 additional information an enzyme-deficient mutant line shows altered flower color, i.e. magenta flowers instead of bright blue due to accumulation of dihydrokaempherol instead of dihydroquercetin 660143 1.14.14.82 additional information an enzyme-deficient mutant line shows altered flower color, i.e. pink flowers instead of dark purple due to accumulation of dihydrokaempherol instead of dihydroquercetin 660143 1.14.14.82 additional information downregulation of the F3'H gene expression by virus-induced gene silencing in soybean does not alter the tawny pubescence pigmentation proved to be unchanged in greenhouse-grown plants, but is reduced when the steady-state mRNA level of the F3'H gene is reduced to approximately 5% of that of greenhouse-grown plants, phenotypes, overview 688651 1.14.14.82 additional information generation of chimeric constructs consisting of cDNA fragments of Gerbera hybrida F3'H and Osteospermum hybrida F3'5'H, EC 1.14.13.88, overview 686737 1.14.14.82 additional information loss of enzyme activity leads to accumulation of 3,5-di-O-(beta-glucopyranosyl)pelargonidin 6''-O-4,6'''-O-1-cyclic malate instead of 3,5-di-O-(beta-glucopyranosyl)cyanidin 6''-O-4,6'''-O-1-cyclic malate, the only difference between these two anthocyanins is a hydroxyl grouppresent at the 3' position in the B-ring of aglycone. Loss of enzme results in a color change in buds from purple to deep pink. Mutation is due to an active hAT type transposable element, designated Tdic101. The color change is attributed to Tdic101 insertion into the second intron of flavonoid 3'-hydroxylase 725984 1.14.14.82 additional information recombinant expression of the flavonoid 3',5'-hydroxylase, EC 1.14.13.88, from Petunia sp. in Dendrathemum grandiflora plants leads to competition of the endogenous flavonoid 3'-hydroxylase with the recombinant enzyme for the same substrates in the flavonoid biosynthetic pathway leading to a change in flower petal colors, phenotype, overview 688630 1.14.14.82 T484F site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme 686737 1.14.14.82 T487A site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme 686737 1.14.14.82 T487S site-directed mutagenesis, a conservative Thr to Ser exchange at position 487 conferred additional 5'-hydroxylation activity to recombinant Gerbera hybrida F3'H 686737 1.14.14.82 V425A mutation in allele 2, complete loss of the ability th hydroxylate the 6'-deoxychalcone isoliquiritigenin in position 3, i.e. reaction of chalcone 3-hydroxylase 744156 1.14.14.82 Y484F/T487S site-directed mutagenesis, altered substrate specificity compared to the wild-type enzyme 686737