2.6.1.62	-	
2.6.1.62	complex of holoenzyme and 7-keto-8-aminopelargonic acid	
2.6.1.62	crystallization of the native and the selenomethionine enzyme, without ligand in two different space groups. In both cases, the structures show a dimer made of two monomers related by a noncrystallographic twofold axis	
2.6.1.62	hanging drop vapor diffusion method	
2.6.1.62	in complex with inhibitors 5-(pyridin-2-yl)thiophene-2-carboxamide, 4-(1H-imidazol-1-yl)benzamide, and N-methyl-1-[4-(1H-pyrazol-1-ylmethyl)phenyl]methanamine	
2.6.1.62	in complex with substrate 7-oxo-8-aminopelargonic acid and in complex with inhibitors 1-(1,3-benzothiazol-2-yl)methanamine and 2-hydrazinyl-1,3-benzothiazole. The side chains of Tyr25, Trp65, Arg400, and Tyr407 are shown to be quite flexible. Small molecule binding induces unexpected conformational remodeling in the substrate binding site	
2.6.1.62	native and the selenomethionine enzyme without ligand, in two different space groups. The structures show a dimer made of two monomers related by a noncrystallographic twofold axis	
2.6.1.62	purified recombinant enzyme, sitting drop vapor diffusion method, mixing of 11 mg/ml protein in 50 mM Tris, 100 mM NaCl, and 0.02 mM PLP, pH 7.9, with precipitant solution containing 0.1 M sodium citrate, pH 6.1, 0.2 M potassium sodium tartrate, and 1.8 M ammonium sulfate. A glass capillary containing 0.008 ml of protein solution is mounted in the gel tube with 1% agarose presoaked in 1 ml of the precipitant solution, method optimization, 1 month at 20°C. X-ray diffraction structure determination and analysis at 1.6 A resolution	
2.6.1.62	purified recombinant mutant enzymes Y17F, Y144F, D147N, R253A, and R253K, hanging drop method, 20°C, 10 mg/ml protein in solution mixed with equal volume of well solution containing 26-28% PEG 4000, 9-12% methylpentanediol, 100 mM HEPES, pH 7.5, microseeding, 2 days, X-ray diffraction structure determination and analysis at 1.7-2.4 A resolution, modeling	
2.6.1.62	to 2.2 A resolution, by molecular replacement, and superimposition of the structures bound either to the S-adenosyl-L-methionine analog sinefungin or to 7-oxo-8-aminopelargonic acid. Comparison to structure of the Bacillus subtilis enzyme, EC 2.5.1.105