2.9.1.2 pyridoxal 5'-phosphate pyridoxal 5'-phosphate is covalently bound to the conserved Lys278 694428 2.9.1.2 pyridoxal 5'-phosphate pyridoxal 5'-phosphate–dependent mechanism of Sec-tRNASec formation. Each SepSecS monomer has a pyridoxal 5'-phosphate cofactor covalently linked to the Nepsilon-amino group of the conserved Lys284 by means of formation of a Schiff base 695136 2.9.1.2 pyridoxal 5'-phosphate the enzyme needs pyridoxal 5'-phosphate to carry out the conversion of O-phosphoserine to selenocysteine 694847