1.2.1.89 NAD+ - 736023 1.2.1.89 NAD+ mutants N286E, N286H, N286T, but not wild-type show activtiy with NAD+ 740187 1.2.1.89 NADH poor cofoactor 736023 1.2.1.89 NADP+ - 762564 1.2.1.89 NADP+ also accepts NAD+ under technically relevant conditions, cofactor above 1 mM, Km for NADP+: 0.022 mM, Km for NAD+ is 22.13 mM, at H 6.2, 50°C 726348 1.2.1.89 NADP+ completely inactive when NAD+ is substituted for NADP+ 724216 1.2.1.89 NADP+ completely inactive with NAD+ 724992 1.2.1.89 NADPH NADPH is preferred over NADH 736023