1.14.13.5 FAD 1 mol per mol enzyme 390102, 390103, 390104, 390105, 390106 1.14.13.5 FAD cannot be replaced by FMN or riboflavin 390104, 390106 1.14.13.5 FAD FAD is the only prosthetic group 390102 1.14.13.5 additional information the enzyme contains 2 mol of titratable suflhydryl groups, one of which is essential, possibly as a substrate-binding site, for the oxygenation of imidazoleacetate 390105 1.14.13.5 NADH - 390101, 390102, 390103, 390104, 390105, 390106 1.14.13.5 NADPH alpha-NADH cannot replace beta-NADH at 0.2 mM 390104 1.14.13.5 NADPH beta-NADPH is approximately 13% as active as beta-NADH 390103, 390104 1.14.13.5 NADPH less efficient than NADH 390102, 390103