4.4.1.11 pyridoxal 5'-phosphate - 34515, 34516, 34518, 34521, 34522, 34530, 34531, 34533, 34534, 34535, 649139, 649323, 649595, 650615, 650770, 651771, 653114, 660621, 660756, 661430, 677329, 680553, 682347, 690268, 691377, 691380, 691957, 692665, 694318, 701880, 714123, 728927, 729181, 729320, 729426, 748205, 748253, 749242 4.4.1.11 pyridoxal 5'-phosphate 4 mol of pyridoxal 5'-phosphate per mole of enzyme 728952 4.4.1.11 pyridoxal 5'-phosphate 4 mol per mol of enzyme 34520, 34523, 34526 4.4.1.11 pyridoxal 5'-phosphate absence of an aldimine bond between the active site Lys210 and pyridoxal 5'-phosphate in enzyme crystals, grown in monomethyl ether polyethylene glycol 2000 in the presence of ammonium sulfate 714356 4.4.1.11 pyridoxal 5'-phosphate increases the half-life of methoxypolyethylene glycol succinimidyl glutarate 5000 treated recombinant METase holoenzyme in a dose-dependent manner 661424 4.4.1.11 pyridoxal 5'-phosphate PLP-binding domain (residues 63-261) is typical for PLP-dependent enzymes with the type I fold 746664 4.4.1.11 pyridoxal 5'-phosphate protein-to-cofactor ratio is 2:1. The cofactor is removable by incubating with hydroxylamine hydrochloride and is readily restored by incubation with free PLP 747532