1.8.1.7 deamino-NADPH 69% of the activity with NADPH 394757 1.8.1.7 FAD - 393993, 394747, 394776, 659698, 674175, 687309, 689958, 696056, 696556, 696681, 697449, 698010, 698440, 699224, 699241, 699614, 699619, 700654, 711000, 711008, 711362, 712066, 712604, 712997, 725689, 726192, 764886 1.8.1.7 FAD 1 mol of FAD per mol of subunit 394740, 394742 1.8.1.7 FAD 1.2 mol per mol of enzyme subunit bound 659902 1.8.1.7 FAD 2 mol of FAD per mol of enzyme 394711, 394717, 394719, 394721, 394722, 394742, 394750, 394753, 394754 1.8.1.7 FAD 4 thiol groups per FAD 393993 1.8.1.7 FAD 5 thiol groups per FAD 393993 1.8.1.7 FAD activity with diverse FAD analogues 394730 1.8.1.7 FAD amino acid sequence of FAD-binding domain 394729 1.8.1.7 FAD color of the purified enzyme is yellow, and it has an absorption spectrum characteristic of flavoproteins 742468 1.8.1.7 FAD each mol of enzyme subunit binds 0.97 mol of FAD 713468 1.8.1.7 FAD FAD enzyme 391653, 394708, 394709, 394711, 394712, 394714, 394721, 394722, 394726, 394727, 394728, 394729, 394730, 394733, 394734, 394735, 394740, 394741, 394742, 394744, 394751, 394752, 394754, 394755, 394760, 394761, 394763, 394765, 394766, 394772, 394775, 394779 1.8.1.7 FAD flavoenzyme, interaction with Asp311 and Leu352, binding domain structure 659698 1.8.1.7 FAD properties of glutathione reductase reconstituted with FAD analogues 394730, 394735 1.8.1.7 FAD required for activity 658730 1.8.1.7 FAD the enzyme contains one FAD per monomer 726208 1.8.1.7 FAD the enzyme possesses the flavin adenine dinucleotide binding motif 764906 1.8.1.7 ferricyanide - 394757 1.8.1.7 flavin - 723817, 726094 1.8.1.7 flavin 1.06 mol of flavin per mol of subunit 394733 1.8.1.7 flavin flavoprotein 394708, 394711, 394737, 394753, 394757 1.8.1.7 additional information as malaria brings riboflavin deficiency, in effection regions a large portion of enzymes miss FAD, but are as well active 394775 1.8.1.7 additional information no activity with FMN 394750, 394752, 394765 1.8.1.7 additional information total lack of activity with 3-acetylpyridine NADH 394757 1.8.1.7 NADH - 394708, 394725, 394758, 394759, 394760 1.8.1.7 NADH 1.3% activity of that with NADPH 394720 1.8.1.7 NADH 10% activity of that with NADPH 394744, 394750 1.8.1.7 NADH 2% activity of that with NADPH 394712 1.8.1.7 NADH 200times less effective than NADPH 394722 1.8.1.7 NADH 20times higher activity with NADH than with NADPH 394732 1.8.1.7 NADH 20times less activity than with NADPH 394708 1.8.1.7 NADH 3% activity of that with NADPH 394755 1.8.1.7 NADH 48fold less activity than with NADPH 394772 1.8.1.7 NADH 8.5% of the activity with NADPH 394757 1.8.1.7 NADH a single enzyme uses both NADPH and NADH as hydrogen donors 394727 1.8.1.7 NADH activity relative to NADPH: 0.5% 394721 1.8.1.7 NADH enzyme can utilize both NADH and NADPH, more active with NADPH 394713 1.8.1.7 NADH less than 1% activity of that with NADPH 394752 1.8.1.7 NADH low activity with NADH 391653, 394724, 394745, 394748, 394767 1.8.1.7 NADH NADH is used as a coenzyme by rat kidney glutathione reductase but with a lower efficiency (32.7%) than NADPH 713468 1.8.1.7 NADH specific for NADH 394751 1.8.1.7 NADPH - 393993, 394708, 394709, 394711, 394712, 394714, 394715, 394716, 394717, 394718, 394719, 394723, 394724, 394726, 394728, 394729, 394730, 394731, 394735, 394736, 394737, 394738, 394739, 394741, 394742, 394743, 394744, 394745, 394746, 394747, 394748, 394749, 394751, 394752, 394754, 394755, 394757, 394760, 394764, 394768, 394769, 394770, 394771, 394773, 394776, 394779, 656625, 657570, 657763, 658730, 658797, 659182, 659484, 659592, 659594, 659902, 660197, 660339, 660485, 675954, 676198, 686077, 686233, 686856, 687309, 688032, 688951, 689306, 689958, 689967, 690147, 694642, 696056, 696556, 696681, 697449, 698010, 698440, 699224, 699241, 699614, 699619, 699801, 699884, 699961, 700654, 700776, 700795, 701018, 701093, 711000, 711008, 711362, 712066, 712604, 712997, 713468, 723817, 724919, 725067, 725616, 725617, 725618, 725689, 725772, 726094, 726192, 726208, 726215, 726354, 726543, 742462, 742468, 742814, 742991, 743274 1.8.1.7 NADPH 200times more activity than with NADH 394722 1.8.1.7 NADPH 20times more activity than with NADH 394708 1.8.1.7 NADPH 48fold more activity than with NADH 394772 1.8.1.7 NADPH 5% of the activity with NADH, 3fold higher affinity of root enzyme compared to chloroplast enzyme 394732 1.8.1.7 NADPH a single enzyme uses both NADPH and NADH as hydrogen donors 394727 1.8.1.7 NADPH absolute specificity for NADPH 394733, 394734, 394740, 394753, 394765, 394766 1.8.1.7 NADPH best electron donor 394720, 394721, 394725, 394778 1.8.1.7 NADPH binding site structure 659698 1.8.1.7 NADPH can utilize both NADPH and NADH, more active with NADPH 391653, 394708, 394713, 394767, 394775 1.8.1.7 NADPH dependent on 657878, 659860 1.8.1.7 NADPH diphosphate group is bound at the C-terminal edge of a parallel stranded beta-sheet as is common in nucleotide-binding proteins 394710 1.8.1.7 NADPH from pentose phosphate pathway 658250 1.8.1.7 NADPH NADPH promoted aggregation of the enzyme 394725 1.8.1.7 NADPH utilizes NADPH but not NADH as electron donor 701146