1.1.1.B4 additional information no cofactor: NADH 740394 1.1.1.B4 additional information no cofactor: NADP+ 687864 1.1.1.B4 additional information the enzyme shows no activity for NADPH or NADP+ 743703 1.1.1.B4 additional information the enzyme shows no activity with NADP(H) 684583 1.1.1.B4 NAD+ - 687864, 688747, 697267, 697839, 723841, 725726, 742305, 743703 1.1.1.B4 NAD+ critical role of the D37 residue in discriminating NAD(H) from NADP(H) 684583 1.1.1.B4 NADH - 687864, 688747, 723841, 725726, 743703 1.1.1.B4 NADH critical role of the D37 residue in discriminating NAD(H) from NADP(H) 684583 1.1.1.B4 NADH preferred over NADPH 721911 1.1.1.B4 NADP+ - 741784 1.1.1.B4 NADPH - 740394, 741784 1.1.1.B4 NADPH about 10% of the activity with NADH 721911 1.1.1.B4 NADPH clear preference for NADPH as cofactor. The phosphate group of NADPH interacts with both the side chains and main-chain amides of Arg38 and Thr15, stabilizing this cofactor significantly better than NADH 711860 1.1.1.B4 NADPH strong preference for NADPH as cofactor 761115