1.1.1.4 4.5 9 activities of Bdh1p(Y) and Bdh1p(B) in glucoladehyde reduction gradually drop to approximately 50% at pH 4.5-7.0 after 6 h incubation. Especially, Bdh1p(Y) still retains 80% of its activity at pH 4.5 after incubation for 3 h. At alkaline conditions (pH 8.0-9.0), activities of the two enzymes drop rapidly to 28-45% within the first 3 h, and 3 more hours later, the lowest activity of Bdh1p(Y) is 17% at pH 9.0 760423 1.1.1.4 5 8.5 stable, rapid denaturation below pH 5.0 721872 1.1.1.4 6 10 stable at 760463 1.1.1.4 7 - purified recombinant His-tagged enzyme, more than 80% residual activity after 18 h at pH 7.0. The activity decreases significantly in acidic circumstances 761565 1.1.1.4 7 7.6 - 246407