2.1.1.11 7.4 - continuous spectrophotometric assay 2.1.1.11 7.5 - assay at 2.1.1.11 7.5 - HPLC-based assay 2.1.1.11 7.7 - phosphate buffer 2.1.1.11 8 - slight preference for Tris/HCl buffer over Tricine/NaOH 2.1.1.11 8.4 - -