4.2.1.22	additional information	-	additional information	the kcat for the generation of H2S by cystathionine beta-synthase of 55/s at 37°C, via the condensation of cysteine and homocysteine is 18fold faster than that for the beta-elimination and rehydration to form serine of 3/s-	714248	
4.2.1.22	0.0045	-	L-cystathionine	reverse reaction (L-cystathionine hydrolysis), mutant S289A	702457	
4.2.1.22	0.022	-	L-cysteine	formation of (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid), mutant E223G, pH 7.5, 37°C	749243	
4.2.1.22	0.024	-	L-homocysteine	recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C	701064	
4.2.1.22	0.031	0.51	L-homocysteine	37°C, pH 8.6, wild-type enzyme	680084	
4.2.1.22	0.04	-	L-cysteine	37°C, pH 8.6, mutant enzyme I278T	680084	
4.2.1.22	0.04	1.97	L-homocysteine	37°C, pH 8.6, mutant enzyme R266K	680084	
4.2.1.22	0.052	2.1	L-serine	37°C, pH 8.6, wild-type enzyme	680084	
4.2.1.22	0.082	-	L-serine	Q157H mutant, pH 8.6, 37°C	650238	
4.2.1.22	0.083	-	L-cystathionine	S82A mutant, pH 8.6, 37°C	650238	
4.2.1.22	0.09	-	L-homocysteine	37°C, pH 8.6, mutant enzyme I278T	680084	
4.2.1.22	0.133	-	L-cystathionine	T85A mutant, pH 8.6, 37°C	650238	
4.2.1.22	0.15	-	L-serine	37°C, pH 8.6, mutant enzyme I278T	680084	
4.2.1.22	0.35	-	H2S	cosubstrate L-serine, pH 7.4, 37°C	746768	
4.2.1.22	0.39	-	L-serine	cosubstrate H2S, pH 7.4, 37°C	746768	
4.2.1.22	0.418	-	L-cystathionine	Y158F mutant, pH 8.6, 37°C	650238	
4.2.1.22	0.45	-	L-serine	T81A mutant, pH 8.6, 37°C	650238	
4.2.1.22	0.52	-	L-serine	37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine	677837	
4.2.1.22	0.56	-	L-cystathionine	pH 8.6, 37°C	650216	
4.2.1.22	0.56	-	L-cystathionine	wild-type enzyme, pH 8.6, 37°C	650238	
4.2.1.22	0.6	-	L-cysteine	formation of L-cystathionine, mutant E223G, pH 7.5, 37°C	749243	
4.2.1.22	0.62	-	L-serine	cosubstrate H2S, presence of S-adenosyl-L-methionine, pH 7.4, 37°C	746768	
4.2.1.22	0.78	-	H2S	cosubstrate L-serine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C	746768	
4.2.1.22	0.85	-	L-homocysteine	cosubstrate: L-serine, mutant S289A	702457	
4.2.1.22	0.85	-	L-serine	cosubstrate: L-homocysteine, mutant S289A	702457	
4.2.1.22	1.03	-	L-cystathionine	reverse reaction (L-cystathionine hydrolysis), wild-type	702457	
4.2.1.22	1.06	-	L-cysteine	cosubstrate H2O, pH 7.4, 37°C	746768	
4.2.1.22	1.1	-	L-cysteine	formation of (2R,2'R)-3,3'-sulfanediylbis(2-aminopropanoic acid), wild-type, pH 7.5, 37°C	749243	
4.2.1.22	1.3	-	L-serine	pH 8.0, 37°C, reaction with cysteamine, continous UV assay	663623	
4.2.1.22	1.41	-	L-cysteine	cosubstrate H2O, presence of S-adenosyl-L-methionine, pH 7.4, 37°C	746768	
4.2.1.22	1.67	-	L-serine	recombinant 6-His-tagged enzyme, in 50 mM Tris (pH 8.6), at 25°C	701064	
4.2.1.22	1.95	-	L-cysteine	37°C, pH 8.6, mutant enzyme R266K	680084	
4.2.1.22	2	-	L-cysteine	formation of L-cystathionine, mutant A70S, pH 7.5, 37°C	749243	
4.2.1.22	2.5	-	L-serine	pH 8.0, 37°C, reaction with cysteamine, Mudd assay	663623	
4.2.1.22	2.9	-	L-serine	37°C, pH 8.6, mutant enzyme R266K	680084	
4.2.1.22	3.13	-	L-cysteine	37°C, pH 8.6, wild-type enzyme	680084	
4.2.1.22	3.13	-	L-cysteine	pH 8.6	665593	
4.2.1.22	3.3	-	L-homocysteine	37°C, pH 8.0, wild-type enzyme, without S-adenosyl-L-methionine	677837	
4.2.1.22	3.38	-	L-homocysteine	wild-type, production of H2S, pH 8.2, 37°C	747747	
4.2.1.22	3.67	-	L-serine	37°C, pH 8.0, wild-type enzyme, without S-adenosyl-L-methionine	677837	
4.2.1.22	3.67	-	L-serine	cosubstrate L-homocysteine, pH 7.4, 37°C	746768	
4.2.1.22	4.39	-	L-cysteine	37°C, pH 8.6, wild-type enzyme	680084	
4.2.1.22	4.39	-	L-cysteine	pH 8.6	665593	
4.2.1.22	4.66	-	L-homocysteine	37°C, pH 8.0, wild-type enzyme, without S-adenosyl-L-methionine	677837	
4.2.1.22	4.66	-	L-homocysteine	cosubstrate L-serine, pH 7.4, 37°C	746768	
4.2.1.22	5.3	-	L-serine	S82A mutant, pH 8.6, 37°C	650238	
4.2.1.22	5.4	-	L-serine	wild-type enzyme, 37°C, absence of S-adenosyl-L-methionine	650017	
4.2.1.22	5.74	-	L-homocysteine	mutant E220R, pH 8.2, 37°C	747747	
4.2.1.22	5.9	-	homocysteine	I435T mutant, 37°C, absence of S-adenosyl-L-methionine	650017	
4.2.1.22	5.9	-	L-serine	I435T mutant, 37°C, absence of S-adenosyl-L-methionine	650017	
4.2.1.22	6.08	-	L-cystathionine	pH 8.6, 37°C	650216	
4.2.1.22	6.08	-	L-cystathionine	wild-type enzyme, pH 8.6, 37°C	650238	
4.2.1.22	6.2	-	homocysteine	wild-type enzyme, 37°C, absence of S-adenosyl-L-methionine	650017	
4.2.1.22	7.09	-	L-cysteine	cosubstrate L-homocysteine, pH 7.4, 37°C	746768	
4.2.1.22	7.38	-	homocysteine	-	5599	
4.2.1.22	7.5	-	L-serine	pH 8.0, 37°C, reaction with L-homocysteine	663623	
4.2.1.22	7.6	-	L-cysteine	formation of L-cystathionine, wild-type, pH 7.5, 37°C	749243	
4.2.1.22	7.6	-	L-serine	37°C	649915	
4.2.1.22	7.93	-	L-homocysteine	37°C, pH 8.6, wild-type enzyme	680084	
4.2.1.22	7.97	-	L-Ser	-	5599	
4.2.1.22	8.17	-	L-homocysteine	cosubstrate L-cysteine, pH 7.4, 37°C	746768	
4.2.1.22	8.2	-	L-serine	Y158F mutant, pH 8.6, 37°C	650238	
4.2.1.22	8.39	-	L-homocysteine	wild-type, pH 8.2, 37°C	747747	
4.2.1.22	9.06	-	L-homocysteine	37°C, pH 8.6, mutant enzyme R266K	680084	
4.2.1.22	10.19	-	L-serine	37°C, pH 8.6, wild-type enzyme	680084	
4.2.1.22	10.2	-	L-serine	pH 8.6	665593	
4.2.1.22	10.91	-	L-homocysteine	mutant A72S, pH 8.2, 37°C	747747	
4.2.1.22	12.7	-	L-homocysteine	37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine	677837	
4.2.1.22	12.7	-	L-homocysteine	cosubstrate L-serine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C	746768	
4.2.1.22	13.2	-	L-serine	T85A mutant, pH 8.6, 37°C	650238	
4.2.1.22	14.01	-	L-serine	37°C, pH 8.0, wild-type enzyme, with S-adenosyl-L-methionine	677837	
4.2.1.22	14.01	-	L-serine	cosubstrate L-homocysteine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C	746768	
4.2.1.22	14.6	-	L-serine	S466L mutant, 37°C, absence of S-adenosyl-L-methionine	650017	
4.2.1.22	14.7	-	L-serine	37°C, L-serine pre-treatment	652270	
4.2.1.22	15.5	-	homocysteine	S466L mutant, 37°C, absence of S-adenosyl-L-methionine	650017	
4.2.1.22	15.8	-	L-serine	I435T mutant, 37°C, presence of S-adenosyl-L-methionine	650017	
4.2.1.22	16.8	-	L-serine	37°C, homocysteine pre-treatment	652270	
4.2.1.22	17	-	L-homocysteine	cosubstrate: L-serine, wild-type	702457	
4.2.1.22	17	-	L-serine	cosubstrate: L-homocysteine, wild-type	702457	
4.2.1.22	19	-	L-serine	pH 6.8, 37°C, deltaC143 mutant after L-serine pre-treatment	649869	
4.2.1.22	19.7	-	L-serine	wild-type enzyme, 37°C, presence of S-adenosyl-L-methionine	650017	
4.2.1.22	21	-	L-serine	pH 6.8, 37°C, full length enzyme after L-serine pre-treatment	649869	
4.2.1.22	21.5	-	L-homocysteine	pH 8.6, 37°C	650216	
4.2.1.22	21.5	-	L-serine	wild-type enzyme, pH 8.6, 37°C	650238	
4.2.1.22	26.78	-	L-homocysteine	cosubstrate L-cysteine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C	746768	
4.2.1.22	31.34	-	L-cysteine	cosubstrate L-homocysteine, presence of S-adenosyl-L-methionine, pH 7.4, 37°C	746768	
4.2.1.22	32.1	-	homocysteine	I435T mutant, 37°C, presence of S-adenosyl-L-methionine	650017	
4.2.1.22	34	-	homocysteine	wild-type enzyme, 37°C, presence of S-adenosyl-L-methionine	650017	
4.2.1.22	39	-	L-serine	pH 6.8, 37°C, full length enzyme after homocysteine pre-treatment	649869	
4.2.1.22	45	-	L-serine	pH 6.8, 37°C, deltaC143 mutant after homocysteine pre-treatment	649869