3.4.21.97 additional information - activation energy below 25°C: 5340 cal/mol 95600 3.4.21.97 4 - gradual degradation, half-life 7 days 95595 3.4.21.97 28 - 10 min 50% loss of activity of mutant enzyme A143Q/S225Y 653320 3.4.21.97 29 - 10 min 50% loss of activity of mutant enzyme A143Q/L229R 653320 3.4.21.97 30 - 10 min 50% loss of activity of mutant enzyme A143Q/D227N and A143Q/S225Y/L229R 653320 3.4.21.97 35 - 10 min, 50% loss of activity of mutant enzymes A143Q and A143Q/R232H 653320 3.4.21.97 37 - 24 h, 40% loss of activity 95608 3.4.21.97 40 - dimeric enzyme is reversibly dissociated 95600 3.4.21.97 50 - partial denaturation 95600 3.4.21.97 52 55 melting temperature of I-site mutant A143V 95603