1.3.8.4 additional information - thermal unfolding analysis 654890 1.3.8.4 48.2 - melting point of mutant A282V without bound ligand 654890 1.3.8.4 48.5 - melting point of mutant A282V with isovaleryl-CoA bound 654890 1.3.8.4 48.6 - melting point of mutant A282V with 2-aza-isovaleryl-CoA bound 654890 1.3.8.4 50.5 - melting point of wild-type enzyme without bound ligand 654890 1.3.8.4 50.9 - melting point of wild-type enzyme with 2-aza-isovaleryl-CoA bound 654890 1.3.8.4 55.4 - melting point of wild-type enzyme with isovaleryl-CoA bound 654890