2.4.2.17 additional information - heat inactivation depends on protein concentration and inhibitors 637668 2.4.2.17 38.9 - melting temperature for subunit HisZ 758806 2.4.2.17 42.9 - melting temperature for subunit HisGS 758806 2.4.2.17 45 - very sensitive to heat inactivation, 0.4 mM histidine stabilizes the enzyme to inactivation by heat 637661 2.4.2.17 47.5 - 1.9 mg enzyme/ml, 80 min, 75% loss of activity without addition of AMP, with 0.05 mM AMP about 60% loss of activity, with 0.5 mM AMP about 40% loss of activity, with 5 mM AMP about 25% loss of activity 637668 2.4.2.17 48 - 3 mg enzyme/ml, 10 min, 15% remaining activity without addition of histidine, with 0.000086 mM histidine about 15% remaining activity after 15 min, with 0.00069 mM histidine about 70% remaining activity after 80 min, with 0.00345 mM histidine about 80% remaining activity after 80 min, with 0.0069 mM histidine about 55% remaining activity after 50 min 637668 2.4.2.17 52.5 - melting temperature of the truncated long-form enzyme devoid of its regulatory domain 758757 2.4.2.17 60.7 - melting temperature of the wild type long-form enzyme 758757