6.3.4.15 additional information - mutant enzymes birA707, birA361 and birA91 with strikingly altered thermosensitivity 6.3.4.15 additional information - melting temperature does not change significantly in presence of ATP, GTP, CTP or UTP 6.3.4.15 55 - melting temperature, BirA-GFP fusion protein. Enzyme is not able to refold after the first cycle of heat denaturation 6.3.4.15 61 - melting temperature, BirA-GFP fusion protein, presence of 1 mM biotin 6.3.4.15 71 - melting temperature, BirA-GFP fusion protein, presence of both 1 mM biotin and 1 mM ATP 6.3.4.15 80 - no denaturation