3.2.1.17 22 - assay at 729765 3.2.1.17 22 - assay at room temperature 729841 3.2.1.17 25 - assay at 718273, 728985 3.2.1.17 25 - assay at, lytic activity, substrate lyophilized cell wall of Micrococcus luteus 729437 3.2.1.17 25 - assay at, substrate Micrococcus lysodeikticus cell walls 729180 3.2.1.17 25 - the optimal assay conditions determined for the recombinant His-tagged protein are in 0.1M potassium phosphate buffer, pH 6.6 containing 1 mM CuCl2 at 25°C 682650 3.2.1.17 30 - - 171051, 677778, 679139 3.2.1.17 30 - assay at 728996, 729766 3.2.1.17 30 - recombinant enzyme 718120 3.2.1.17 30 40 substrate: lyophilized cell wall of Micrococcus luteus 664365 3.2.1.17 30 50 below 30°C and above 50°C is a sharp decrease of activity 751265 3.2.1.17 35 - lytic activity towards Micrococcus lysodleikticus 679957 3.2.1.17 37 - - 171081, 680003, 729763 3.2.1.17 37 - assay at 654265, 656731, 718309, 718362, 729871, 730185 3.2.1.17 37 - optimum temperature for isopeptidase activity 750569 3.2.1.17 37 42 assay at 717301 3.2.1.17 40 - - 700107, 749613, 751934 3.2.1.17 40 - assay at 171093, 171094 3.2.1.17 40 - FII 171095 3.2.1.17 40 - recombinant enzyme 718276 3.2.1.17 40 - substrate: lyophilized cell wall of Micrococcus luteus 664365 3.2.1.17 40 45 - 664651 3.2.1.17 42 - assay at, substrate 4-methylumbelliferyl-tetra N-acetyl-beta-chitotetraoxide 729180 3.2.1.17 45 - - 171041 3.2.1.17 50 - - 171079, 679932, 679934 3.2.1.17 50 - assay at 729432 3.2.1.17 50 - assay at, substrate N-acetylglucosamine oligomers 729437 3.2.1.17 50 - FI 171095 3.2.1.17 50 - the relative activity decreases above 50°C 751489 3.2.1.17 60 - - 171040, 679934, 752907 3.2.1.17 60 - lytic activity 729619 3.2.1.17 60 - lytic activity against Micrococcus lysodeikticus 729620 3.2.1.17 65 - immobilized enzyme 730842 3.2.1.17 75 - - 729775