3.5.1.116 expressed in Nicotiana benthamiana leaf epidermal cells 3.5.1.116 recombinant expression of seleno-L-methionine-substituted, N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 3.5.1.116 recombinant expression of wild-type seleno-L-methionine-substituted, N-terminally His-tagged enzyme and enzyme mutants in Escherichia coli strain BL21(DE3)