3.4.21.63 gene prtA, DNA and amino acid sequence determination and analysis, expression in Pichia pastoris strain X-33 as inactive enzyme, the presence of this enzyme was toxic for the host and resulted in cell lysis, modification of the culture medium constituents improve the expression by 6.4fold 731156 3.4.21.63 gene sapHM, DNA and amino acid sequence determination and analysis, phylogenetic tree 754408 3.4.21.63 high-level expression of plasmids pPIC9K-n/Alp and pPIC9K-alpha/Alp in Pichia pastoris GS115 with native signal peptide or alpha-factor secretion signal peptide 682690 3.4.21.63 overexpression in Escherichia coli strain BL21(DE3) 717658 3.4.21.63 recombinant expression of a truncated neutral protease I in Pichia pastoris with a high enzyme yield 731961 3.4.21.63 recombinant expression of His6-tagged enzyme without signal peptide in Pichia pastoris strain KM71 755309 3.4.21.63 sequence comparisons between Aspergillus species and different proteases, clustering of alkaline proteases, phylogenetic tree, overview 717111 3.4.21.63 strain U1521 contains multiple copies of alkaline protease gene 652661