3.1.27.8	expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)	
3.1.27.8	recombinant expression of C-terminally FLAG-V5-His6-tagged enzyme in HEK293 cell, recombinant expression of N-terminally GST-tagged wild-type and mutant enzymes in Escherichia coli