2.4.1.230 - 2.4.1.230 DNA and amino acid sequence determination and analysis, expression in Escherichia coli 2.4.1.230 expression of His-tagged LaMP loop 3 mutants in Escherichia coli strain BL21(DE3) 2.4.1.230 heterologous expression in Escherichia coli 2.4.1.230 kojibiose phosphorylase gene and trehalose phosphorylase gene are intracellularly hyperexpressed under the control of the Bacillus amyloliquefaciens alpha-amylase promoter in Bacillus subtilis 2.4.1.230 recombinant expression of wild-type and mutant enzymes