1.3.8.6 DNA and amino acid sequence determination annd analysis, phylogenetic tree, expression with a cleavable 6-His fusion tag followed by the human rhinovirus 3C protease-cleavage sequence in Escherichia coli strain BL21 (DE3) 1.3.8.6 expressed in Escherichia coli 1.3.8.6 expressed in Escherichia coli D5alpha and MC4100 cells 1.3.8.6 expressed in the Azoarcus sp. CIBdgcdR mutant strain 1.3.8.6 expression of wild-type and mutant enzymes in Escherichia coli 1.3.8.6 GCDH genotyping in black South African population, overview 1.3.8.6 recombinant expression of C-terminally His6-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 1.3.8.6 wild type and mutant of patient with glutaric acidemia type I