1.3.1.3 DNA and amino acid sequence determination and analysis, sequence comparisons, expression of wild-type and mutant enzymes in Escherichia coli strain M15 1.3.1.3 expressed in Escherichia coli 1.3.1.3 expressed in Escherichia coli strain C41(DE3) 1.3.1.3 expressed in Escherichia coli strain M15 1.3.1.3 expression in Escherichia coli 1.3.1.3 expression in Escherichia coli and HEK-293 cell 1.3.1.3 expression in human HEK-293 cells 1.3.1.3 expression of GST-tagged enzyme in Escherichia coli 1.3.1.3 expression of splice variant AKR1D1 in HEK-293 cell 1.3.1.3 full-length P5betaR cDNA overexpressed in Escherichia coli as a fusion protein with MBP or His-tag