1.3.1.102 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His-tagged isozyme PaDBR1 in Escherichia coli strain BL21(DE3) 744897 1.3.1.102 DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant expression of N-terminally His-tagged isozyme PaDBR2 in Escherichia coli strain BL21(DE3) 744897 1.3.1.102 gene aor, real-time PCR. enzyme expression analysis 746111 1.3.1.102 gene cloned into pET21b (Novagen), without a stop codon, to incorporate a C-terminal His6-tag and transformed Escherichia coli strain BL21(DE3)pLysS (Stratagene) 721126 1.3.1.102 NtRed-1 cloned in Escherichia coli 685505 1.3.1.102 quantitative RT-PCR enzyme expression analysis 746176