1.2.1.88 - 1.2.1.88 expressed in Escherichia coli BL21(DE3) cells 1.2.1.88 expression in Escherichia coli 1.2.1.88 gene ALDH12, composed of 15 exons on chromosome 6, phylogenetic analysis, the maize cultivar Cellux cDNA sequence differs in 15 bases leading to five amino acid substitutions (P86S, V88M, A127T, V468I and V532I) compared to the sequenced B73 cultivar, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli 1.2.1.88 gene ALDH12, composed of 16 exons on chromosome 5, phylogenetic analysis 1.2.1.88 into the pGEM-T easy vector for sequencing 1.2.1.88 prn gene cluster characterization with help of mutations and physical mapping 1.2.1.88 pruA gene, nucleotide sequence and deduced amino acid sequence thereof