1.14.13.50 - 1.14.13.50 analogues of PCP catabolic genes (cpsABCDE, and p450) are successfully amplified from the whole genome of BtAOA using the primers designed based on the gene sequences retrieved from the whole genome annotation data submitted at NCBI 1.14.13.50 DNA and amino acid sequence determination and analysis, expression of His-tagged wild-type and mutant enzymes in Escherichia coli 1.14.13.50 Escherichia coli M15 overexpressing recombinant hexahistidine-tagged pentachlorophenol 4-monooxygenase 1.14.13.50 expression in Escherichia coli 1.14.13.50 expression of wild-type and mutant enzymes in Escherichia coli strain BL21(DE3) 1.14.13.50 gene isolated from Pseudomonas sp. plasmid, recombinant expression in Escherichia coli XLBlue1alpha, DNA sequence analysis of the recombinant clones, comparison with other sequences 1.14.13.50 gene pcpB, DNA and amino acid sequence analysis, phylogenetic analysis 1.14.13.50 gene pcpB, expression in Escherichia coli 1.14.13.50 gene pcpD, expression as C-terminally His12-tagged protein in Pseudomonas aeruginosa strain PAO1-Lac