2.7.1.73 - 2.7.1.73 gene gsk, recombinant expression in Corynebacterium ammoniagenes ATP-regenerating strain ATCC 21477, the Escherichia coli trp promoter proves to be most efficient with regard to inducing the expression of gsk, as compared to tac promoter 2.7.1.73 gene gsk, several metabolic enzymes such as GMP synthetase and IMP dehydrogenase, GMP reductase, and guanosine-inosine kinase, all involved in the biosynthesis of GMP, are co-overexpressed in recombinant Escherichia coli TOP10 and BL21star(DE3) cells becoming able to produce GDP-L-fucose, pH-stat fed-batch fermentations quantification and optimization, overview