2.6.1.97 expressed in Escherichia coli BL21(DE3) cells 765740 2.6.1.97 gene arcS, recombinant expression of enzyme ArcS in an arcS orthologue (KEGG accession TK2156)-deletion strain of Thermococcus kodakarensis and functional complementation 759819 2.6.1.97 gene mj1022 719859 2.6.1.97 gene tgtA2 719859 2.6.1.97 in the Escherichia coli K12 MG1655 strain, the queF and queC are deleted (DELTAque/DELTAqueF), and the resulted deletion strain is transformed with an expression plasmid containing GAT-queC from S. solfataricus (SSO0016) cloned behind a PBAD promoter 717018 2.6.1.97 recombinant coexpression of His-tagged enzyme in Escherichia coli strain ER2566 759819 2.6.1.97 recombinant coexpression of His-tagged TaArcS and His-tagged TkRaSEA in Escherichia coli 759819