2.3.1.255 - 705613 2.3.1.255 a plasmid expressing hNaa16p, NARG1L-FLAG, is used 702870 2.3.1.255 cloning of V5-tagged hNaa15p, hNaa25p, and hNaa35p from HEK-293 cell genomic DNA, expression of His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p in Escherichia coli, efficient expression of MBP-hNaa15p requires coexpression of pDC952, a plasmid carrying the Escherichia coli argU gene 720453 2.3.1.255 expressed in Escherichia coli BL21 cells 735797 2.3.1.255 expressed in Escherichia coli BL21(DE3) cells 730955 2.3.1.255 expressed in Escherichia coli BL21(DE3)pLysS cells 739801 2.3.1.255 expressed in Escherichia coli Rosetta (DE3)pLysS cells 736506 2.3.1.255 expressed in Escherichia coli Rosetta II(DE3)pLysS cells 739801 2.3.1.255 expressed in Escherichia coli Rosetta Star cells 741164 2.3.1.255 gene NAA10, DNA and amino acid sequence determination and analysis, genotyping, recombinant expression of His-MBP-tagged wild-type and mutant Naa10 in Escherichia coli strain BL21 Star DE3 756298