2.3.1.255 - 2.3.1.255 a plasmid expressing hNaa16p, NARG1L-FLAG, is used 2.3.1.255 cloning of V5-tagged hNaa15p, hNaa25p, and hNaa35p from HEK-293 cell genomic DNA, expression of His-tagged and maltose binding protein-tagged subunits hNaa15p, hNaa10p, and hNaa50p in Escherichia coli, efficient expression of MBP-hNaa15p requires coexpression of pDC952, a plasmid carrying the Escherichia coli argU gene 2.3.1.255 expressed in Escherichia coli BL21 cells 2.3.1.255 expressed in Escherichia coli BL21(DE3) cells 2.3.1.255 expressed in Escherichia coli BL21(DE3)pLysS cells 2.3.1.255 expressed in Escherichia coli Rosetta (DE3)pLysS cells 2.3.1.255 expressed in Escherichia coli Rosetta II(DE3)pLysS cells 2.3.1.255 expressed in Escherichia coli Rosetta Star cells 2.3.1.255 expression in HeLa cells