4.2.1.134 expressed in Nicotiana benthamiana and Arabidopsis thaliana epidermal cells 720893 4.2.1.134 expressed in Saccharomyces cerevisiae strain SAY32 719491 4.2.1.134 gene MTH12.2 or At5g59770, expression analysis, PTPLA is fused with CYFP- or NYFP- at the N-terminal of the protein prevent potential interference with ER retention signal located at the C-terminal end, transient expression of 35S:mCherry-PTPLA constructs in Nicotiana benthamiana. The subcellular distribution of mCherry-PTPLA shows the characteristic endoplasmic reticulum network which is confirmed by its colocalization with endoplasmic reticulum-localized GFP-PAS2 fusion, in contrary to the PTPLA promoter, the KCR2 promoter is also expressed in cotyledons, leaves and in the meristem of secondary roots 749092 4.2.1.134 gene PAS2 or At5g10480, expression analysis, transient expression of GFP-PAS2 fusion constructs in Nicotiana benthamiana endoplasmic reticulum 749092 4.2.1.134 gene PH, de novo assembly and functional annotation of Aurantiochytrium transcriptome and library screening, sequence comparisons, quantitative real-time PCR expression analysis, gene PH expression pattern analysis 748533 4.2.1.134 gene phs1, encoding 3-hydroxyacyl-CoA dehydratase required for the third step in very long chain fatty acid biosynthesis, the Sporobolomyces sp. PHS1 gene complements the essential functions of the Saccharomyces cerevisiae phs1 loss-of-function mutant strain phs1::KanMX/PHS1, transfection using the Agrobacterium tumefaciens system, lipid analysis of the strains 749134