4.2.1.104 - 4.2.1.104 expressed in Escherichia coli 4.2.1.104 expressed in Escherichia coli, cyanase gene cynS not arranged in a three-gene cluster 4.2.1.104 expression in Escherichia coli DH5 alpha 4.2.1.104 expression in Escherichia coli strain M15 4.2.1.104 gene Atcyn, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21 (DE3) as His-tagged soluble protein 4.2.1.104 gene cynS, cynS is presumably transcribed as part of the cynABDS operon, DNA and amino acid sequence determination and analysis, phylogenetic analysis 4.2.1.104 gene cynS, DNA and amino acid sequence determination and analysis 4.2.1.104 gene cynS, DNA and amino acid sequence determination and analysis, phylogenetic analysis, overexpression in Escherichia coli strain HMS174 as maltose-binding-protein fusion protein 4.2.1.104 gene cynS, DNA and amino acid sequence determination and analysis, tightly clustered with 2 genes located upstream, which encode proteins similar to the subunits of nitrate-nitrite transporter, complementation of deficient Escherichia coli strain 4.2.1.104 gene cynS, functional recombinant expression of C-terminally His-tagged enzyme in Arabidopsis thaliana plants ecotype Columbia under control of the CaMV 35S promoter via the Agrobacterium tumefaciens (GV3101)-mediated floral dip transformation method, ammonia release assay, pigment contents, and total carbohydrate measurements of transgenic plants, overview 4.2.1.104 gene cynS, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3) or Trichoplusia ni High Five cells 4.2.1.104 gene cynS, tightly clustered with 4 putative molybdenum cofactor biosynthesis genes located downstream 4.2.1.104 gene Oscyn, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression in Escherichia coli strain BL21 (DE3) as His-tagged soluble protein 4.2.1.104 gene tetur28g02430, DNA and amino acid sequence determination and analysis, genomic and transcriptomic analysis, phylogenetic analysis, recombinant expression of MBP-tagged or N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 4.2.1.104 gene tetur28g02430, sequence comparisons and phylogenetic analysis, expression of His-tagged enzyme in Escherichia coli BL21(DE3) pLys, the Tetranychus urticae cyanase remains functionally active after horizontal gene transfer