3.5.4.38 - 3.5.4.38 construction of stable transformants (mouse hybridoma cells) expressing human activation-induced cytidine deaminase 3.5.4.38 expressed in Escherichia coli 3.5.4.38 expression HEK 293T cells 3.5.4.38 expression in Escherichia coli 3.5.4.38 expression in Escherichia coli as a glutathione S-transferase fusion protein 3.5.4.38 expression in Escherichia coli strain C43(DE3)pLysS 3.5.4.38 expression in Escherichia coli. Human activation-induced cytidine deaminase purified from Escherichia coli is active without prior treatment with a ribonuclease and deaminates cytosines in plasmid DNA in vitro 3.5.4.38 expression in HeLa cell and HEK 293T cell 3.5.4.38 expression in Sf9 cells 3.5.4.38 expression of 3H9 VDJ knock-in alleles in genomic DNA of immature/T1, mature follicular B cells, and thymocytes from 3H9 and 3H9.Aicda-/- mice, quantitative expression analysis by PCR and immunofluorescence