3.5.4.13 - 3.5.4.13 expressed in Escherichia coli BL21(DE3) cells 3.5.4.13 expression in Escherichia coli 3.5.4.13 ORF A596R, DNA and amino acid sequence determination and analysis, expression of the His6-tagged enzyme in Escherichia coli strain DH5MCR, phylogenetic analysis