3.4.21.81 active-site variant genes clones in the Escherichia coli/Bacillus subtilis shuttle vector pEB-11 and expressed in Bacillus subtilis DB104 3.4.21.81 gene structure 3.4.21.81 genes constructed which encodes deletions at the amino-terminal end of the propeptide, deletions to the 76-amino-acid propeptide of SGPB cause an incremental loss in the production of the mature enzyme