3.4.21.77 - 3.4.21.77 expressed in Escherichia coli M15(pREP4) cells 3.4.21.77 expressed in HUVEC cells 3.4.21.77 gene hK3 or KLK3, genetic organization and structure of the kallikrein gene family, clustered on chromosome 19q13.3-q13.4 3.4.21.77 gene structure 3.4.21.77 male C57BL/6 mice injected with recombinant adenoviruses containing rAd/PSMA, rAd/PSCA and rAd/STEAP 3.4.21.77 proPSA and active PSA cDNA cleaved with EcoRI and HindIII and cloned into vector pET28(a)+, expressed in Escherichia coli strain BL21(DE3). Recombinant vector cleaved with EcoRI and XhoI, inserted into vector pGEX4T-1 and expressed in Escherichia coli strain BL21(DE3) 3.4.21.77 recombinant proteins of proPSA and active PSA cloned into pGEX4T-1 expression vector that produces recombinant proteins with N-terminal GST tags. The proteins expressed in Escherichia coli BL21(DE3) 3.4.21.77 structure of the gene and linkage to the kallikrein-like gene hGK-1