3.4.16.2	-	
3.4.16.2	a cDNA fragment encoding human pro-CPA6 is subcloned into the pcDNA3.1 vector	
3.4.16.2	cDNA cloned in pMT/BIP/V5-HIS-C, transfected into Schneider insect cells	
3.4.16.2	cloned in pMT/BIP/V5-HIS-C, transfected into Scheider insect cells	
3.4.16.2	expressed in AT-20 cells	
3.4.16.2	expressed in Schneider insect cells	
3.4.16.2	overexpression in Chineses hamster ovary cells. CHO cells are transfected with full-length prolylcarboxypeptidase under the control of a cytomegalovirus promoter. CHO recombinant prolylcarboxypeptidase is expressed as a fusion protein with COOH-terminal enhanced green fluorescence protein. Overexpression in the external membrane	
3.4.16.2	PrCP is cloned with an N-terminal FLAG tag and a C-terminal heptahistidine tag under the trypsin II signal sequence in pcDNA5/FRT and expressed in stably transfected CHO cells