3.2.1.68	-	136799, 654414, 656966, 657126	
3.2.1.68	cloned to an expression vector with a T7lac promoter. Both wild-type and His-tagged isoamylases are expressed in Escherichia coli	666093	
3.2.1.68	cloning of cDNAs of different isoforms, Psisa1-Psisa3, DNA and amino acid sequence determination and analysis, phylogenetic analysis and sequence comparisons, overview	710572	
3.2.1.68	coexpression of wild-type His-tagged ISA1 and ISA2, and of mutant D367A ISA1 with wild-type ISA2, in Escherichia coli strain BL21(DE3)	732750	
3.2.1.68	DNA and amino acid sequence determination and analysis, phylogenetic tree	732278	
3.2.1.68	DNA and amino acid sequence determination and analysis, sequence comparisons	732581	
3.2.1.68	expressed in Escherichia coli	713710	
3.2.1.68	expressed in Escherichia coli BL21 cells	751948	
3.2.1.68	expressed in Escherichia coli BL21(DE3) cells	716495, 750238, 751254	
3.2.1.68	expressed in Escherichia coli BL21(DE3) Star cells	716622	
3.2.1.68	expressed in Escherichia coli strain MDS42	749623	
3.2.1.68	expressed in Saccharomyces cerevisiae	713710	
3.2.1.68	expression in Escherichia coli	665267	
3.2.1.68	expression of in Escherichia coli	672691	
3.2.1.68	expression of OsISA2 in Escherichia coli	682469	
3.2.1.68	expression of OsISA2in Escherichia coli	682469	
3.2.1.68	expression of wild-type and mutant enzymes in Escherichia coli, sequence comparison	693147	
3.2.1.68	gene ArDBE, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis	732490	
3.2.1.68	gene isa1, single expression of C-terminally His8-tagged subunit in Escherichia coli strain BL21(DE3), co-expression of untagged ISA1 subunit with C-terminally His8-tagged subunit ISA2 in Escherichia coli	732655	
3.2.1.68	gene isa2, single expression of C-terminally His8-tagged subunit in Escherichia coli strain BL21(DE3) or co-expression with untagged ISA1 subunit in Escherichia coli	732655	
3.2.1.68	gene su-1, single expression of C-terminally His8-tagged subunit ISA1 in Escherichia coli strain BL21(DE3), co-expression of untagged ISA1 subunit with C-terminally His8-tagged subunit ISA2 in Escherichia coli. Zea mays ISA1 subunit is heterologously expressed in Arabidopsis thaliana lacking endogenous ISA1 or lacking both native ISA1 and ISA2 via transfection with Agrobacterium tumefaciens strain GV3101. The maize protein functions in Arabidopsis leaves to support nearly normal starch metabolism in the absence of any native ISA1 or ISA2	732655	
3.2.1.68	gene su1, expressed in Escherichia coli, pAR4	136814	
3.2.1.68	gene treX, component of a treXYZ operon, DNA and amino acid sequence determination and analysis, sequence comparison, genetic organization in the treXYZ operon, overview	717269	
3.2.1.68	iam gene cloned and expressed in Escherichia coli JM101, pMON17481, production of a nonsecreted signal peptide-less isoamylase	136813	
3.2.1.68	into Agrobacterium tumefaciens strain GV310, GFP/GUS promoter fusion constructs for AtISA1, AtISA2, AtISA3 into Arabidopsis thaliana using the floral dip method	681140	
3.2.1.68	introduction of ISA1 gene from Triticum aestivum (TaISA1 from the D genome donor Aegilops tauschii) into a rice sugary-1 mutant line EM914 in which starch is completely replaced by phytoglycogen in the endosperm	666629	
3.2.1.68	isoamylase gene is a single copy in rice genome, located on chromsome 8	136815	
3.2.1.68	plasmid pIAM275, pUC9 carrying iam gene, Escherichia coli, recominant plasmid does not direct the synthesis of isoamylase in Escherichia coli , sequenced	136807	
3.2.1.68	recombinant GST-ISA2 fusion protein is expressed in Escherichia coli Rosetta DE3-pLysS cells	716600	
3.2.1.68	the STA7 locus encodes ISA1, recombinant expression of His-tagged subunit ISA1 in Escherichia coli strain BL21(DE3)	732146	
3.2.1.68	the STA8 locus encodes ISA2, recombinant expression of HA-tagged ISA2 in strain BafV13 and BafO6	732146