3.2.1.139 Escherichia coli BL21(DE3)pLysE, Luria-Bertani broth, 37°C, plasmid pET29-Gste-AG-his-S 695680 3.2.1.139 expressed in Escherichia coli Rosetta(DE3) cells 701796 3.2.1.139 expressed in Escherichia coli Tuner (DE3) cells 713669 3.2.1.139 expressed in Pichia pastoris strain X33 745060 3.2.1.139 expression in Escherichia coli 26110, 664345, 664349, 664926, 738687 3.2.1.139 expression in Pichia pastoris 737907 3.2.1.139 expression in Saccharomyces cerevisiae 664800 3.2.1.139 expresssion in Arabidopsis thaliana 737933 3.2.1.139 from strain A1163, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant expression of His-tagged enzyme in Pichia pastoris 731869 3.2.1.139 gene deg75-AG, DNA and amino acid sequence determination and analysis, recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3)pLysE 732277 3.2.1.139 into pGEM-T Easy vector, plasmids maintained in Escherichia coli JM109 679583 3.2.1.139 overexpression in Aspergillus niger 737908 3.2.1.139 overexpression in Escherichia coli using the T7 polymerase expression system 646735 3.2.1.139 Pichia stipitis CBS 6054 grown in YNB medium, cells washed and suspended in YNB medium with a xylooligosaccharide misture and methyl-beta-xylopyranoside, 30°C, 24 h, shaker 697944 3.2.1.139 recombinant expression in Saccharomyces cerevisiae strain MH1000pbk, optimisation and scale-up of enzyme production in aerobic fed-batch culture with constant growth rate, strain growth parameters at at 30°C and pH 5.5, and growth and production profiles , overview. The enzyme is secreted into the culture supernatant 731265 3.2.1.139 recombinant expression of His6-tagged enzyme in Escherichia coli strain BL21(DE3) 732167 3.2.1.139 the gene is isolated from a rumen metagenomic library 732877