3.1.6.4 expressed in deficient fibroblasts 651453 3.1.6.4 expression in Chinese hamster ovary cells 135654 3.1.6.4 expression in Escherichia coli 752227 3.1.6.4 expression in Escherichiacoli BL21 730183 3.1.6.4 expression in MCF-7 cells. Following overexpression of the enzyme, total sulfated glycosaminoglycans, chondroitin 4-sulfate, and chondroitin sulfates decline significantly 680928 3.1.6.4 expression in Pichia pastoris 752199 3.1.6.4 overexpression in chinese hamster ovary cells 681958 3.1.6.4 recombinant enzyme expression in Escherichia coli strain BL21 in both soluble and inclusion bodies fractions, rGALNS amounts in inclusion bodies fraction are up to 17fold higher than those observed in the soluble fraction, method optimization, effect of aeration and agitation at bench scale, detailed overview 715797 3.1.6.4 the GALNS gene is located on chromosome 16q24.3, DNA and amino acid sequence determination and analysis of wild-type and mutant genes 666376