3.1.3.75	-	
3.1.3.75	expressed in Escherichia coli BL21	
3.1.3.75	expressed in Escherichia coli BL21(DE3)	
3.1.3.75	expressed in Escherichia coli ER2566 cells	
3.1.3.75	expression in Escherichia coli TOP10	
3.1.3.75	expression of His-tagged wild-type and mutant fragment comprising residues M19-C267 in Escherichia coli	
3.1.3.75	expression of wild-type and mutant enzymes in Escherichia coli strain BL21	
3.1.3.75	from growth plate chondrocytes, expression in Escherichia coli TOP10	
3.1.3.75	gene PA5292, expression in Escherichia coli, expression in and complementation of a Pseudomonas aeruginosa mutant strain JUF8-00	
3.1.3.75	gene pchP, expression of His-tagged enzyme in Escherichia coli strain BL21	
3.1.3.75	gene pchP, phylogenetic tree, regulation of pchP gene expression is under the control of GbdR and NtrC. PchP expression in Escherichia coli as an N-terminal fusion to intein or a histidine tag	
3.1.3.75	gene pchP327, recombinant expression with a N-terminal fusion to an MGSSHHHHHHSSGLVPRGSH tag in Escherichia coli BL21, expression of N-terminal His6-tagged PchP in Escherichia coli	
3.1.3.75	His-tagged version expressed in Escherichia coli BL21 Codonplus	
3.1.3.75	into vector pBAD TOPO TA, expressed in Escherichia coli	
3.1.3.75	overexpression of the wild-type and mutant enzymes in Escherichia coli ER2566, transformed with pTY-pchP327, pTYpchP349, or the mutated expression plasmids	
3.1.3.75	sequencing