2.9.1.2 - 2.9.1.2 expressed in Escherichia coli SelA-deficient strain JS1 2.9.1.2 phylogenetic analysis and tree 2.9.1.2 gene SEPSECS, recombinant expression of His6-tagged enzyme mutants in Escherichia coli strain BL21(DE3) and SoluBL21(DE3) (a bacterial strain engineered to increase solubility of recombinant proteins)