2.8.1.6 - 2.8.1.6 expressed in Escherichia coli B834(DE3) cells 2.8.1.6 expressed in Escherichia coli BL21(DE3)pLysS cells 2.8.1.6 expression in Escherichia coli 2.8.1.6 expression in Escherichia coli. The amino-terminal portion of the BIO2 gene may play a role in localizing the BIO2 protein to a subcellular compartment 2.8.1.6 hexahistidine tagged protein is expressed in Escherichia coli cells 2.8.1.6 hexahistidine tagged proteins are expressed in Escherichia coli BL21DE3 pLysS cells 2.8.1.6 The delta pdxH::CAT allele of strain TX2767 is transduced into strain ER47 with phage P1vir to give strain AH14 into which plasmids pER30 and pER35 are introduced 2.8.1.6 the primary sequence of the Arabidopsis biotin synthase is similar to biotin synthase from Escherichia coli, Serratia marcescens and Saccharomyces cerevisiae, about 50% sequence identity, and more distantly related to the Bacillus sphaericus enzyme, 33% sequence identity 2.8.1.6 wild-type and mutants