2.7.4.27 His-tagged and GFP-fusion proteins expressed in Escherichia coli BL21(DE3) 2.7.4.27 His-tagged protein expressed in Escherichia coli NM522 2.7.4.27 recombinant overexpression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) RIL 2.7.4.27 recombinant overexpression of wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3) RIL