2.6.1.97 expressed in Escherichia coli BL21(DE3) cells 2.6.1.97 gene arcS, recombinant expression of enzyme ArcS in an arcS orthologue (KEGG accession TK2156)-deletion strain of Thermococcus kodakarensis and functional complementation 2.6.1.97 gene mj1022 2.6.1.97 gene tgtA2 2.6.1.97 in the Escherichia coli K12 MG1655 strain, the queF and queC are deleted (DELTAque/DELTAqueF), and the resulted deletion strain is transformed with an expression plasmid containing GAT-queC from S. solfataricus (SSO0016) cloned behind a PBAD promoter 2.6.1.97 recombinant coexpression of His-tagged enzyme in Escherichia coli strain ER2566 2.6.1.97 recombinant coexpression of His-tagged TaArcS and His-tagged TkRaSEA in Escherichia coli