2.3.1.75 - 659305, 735489, 737286 2.3.1.75 chromosomal location, expression of the FLAG-tagged enzyme in Spodoptera frugiperda Sf9 cells and in COS-7 cells 659670 2.3.1.75 cloning of cDNA, DNA sequence analysis, construction of plasmid to express the cDNA in transgenic Arabidopsis thaliana plants 487487 2.3.1.75 delta integration in Saccharomyces cerevisiae, resulting in 1–6 copies of the integration construct per genome 735806 2.3.1.75 DNA and amino acid sequence determination and analysis of wax synthase analogous genes, expression patterns, overview 706345 2.3.1.75 expressed in Arabidopsis thaliana fad2 fae1 double mutant 756250 2.3.1.75 expressed in Escherichia coli C41(DE3) cells 755845 2.3.1.75 expressed in Escherichia coli Rosetta (DE3) cells and Saccharomyces cerevisiae quadruple mutant H1246 756245 2.3.1.75 expressed in Saccharomyces cerevisiae 757376 2.3.1.75 expressed in Saccharomyces cerevisiae quadruple mutant strain H1246 743538, 755872 2.3.1.75 expressed in Saccharomyces cerevisiae strain H1246 758397 2.3.1.75 expressed in seeds of Arabidopsis thaliana and Camelina sativa 757957 2.3.1.75 expression as soluble N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) 657612 2.3.1.75 expression in Escherichia coli 718651 2.3.1.75 expression in Saccharomyces cerevisiae 720358, 735353 2.3.1.75 expression in Saccharomyces cerevisiae and Escherichia coli 689634 2.3.1.75 functional expression in Escherichia coli strain BL21(DE3) 671446 2.3.1.75 gene atfA, DNA and amino acid sequence determination and analysis, WS/DGAT family sequence comparisons and phylogenetic relationships, overview, expression in Escherichia coli as untagged or C-terminally His6-tagged enzyme 672451 2.3.1.75 gene atfA, expression of wild-type and mutant enzymes in Escherichia coli strain Rosetta(DE3) 703547 2.3.1.75 gene atfG25, DNA and amino acid sequence determination and analysis, genomic structure, sequence comparisons, recombinant expression of His-tagged enzyme in Escherichia coli strain C41(DE3) 755845 2.3.1.75 gene AWAT1, i.e. DGA2, location on the X chromosome Xq13.1, DNA and amino acid sequence determination and analysis, expression analysis, expression in Saccharomyces cerevisiae 674424 2.3.1.75 gene DC3, DNA and amino acid sequence determination and analysis, genetic organization and phylogenetics, expression in Saccharomyces cerevisiae 674424 2.3.1.75 gene DC4, DNA and amino acid sequence determination and analysis, genetic organization and phylogenetics, expression in Saccharomyces cerevisiae 674424 2.3.1.75 gene DC4, i.e. AWAT2, location on the X chromosome Xq13.1, DNA and amino acid sequence determination and analysis, expression analysis, expression in Saccharomyces cerevisiae 674424 2.3.1.75 gene DGAT2, DNA and amino acid sequence determination and analysis, genetic organization and phylogenetics, expression in Saccharomyces cerevisiae 674424 2.3.1.75 gene WSD2, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons and phylogenetic analysis, analysis of the molecular organization of six WSD isoforms in Euglena gracilis, quantitative PCR enzyme expression analysis in wild-type and mutant cells 758397 2.3.1.75 gene WSD4, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast 756245 2.3.1.75 gene WSD5, DNA and amino acid sequence determination and analysis, isozyme sequence comparisons and phylogenetic analysis, analysis of the molecular organization of six WSD isoforms in Euglena gracilis, quantitative PCR enzyme expression analysis in wild-type and mutant cells 758397 2.3.1.75 gene WSD5, phylogenetic analysis, recombinant expression of C-terminally His6-tagged enzyme in Escherichia coli strain Rosetta (DE3), recombinant expression in TAG-deficient Saccharomyces cerevisiae mutant strain H1246 results in the accumulation of wax esters, but not any detectable triacylglycerols (TAGs), indicating a predominant wax synthase (WS) activity in yeast 756245 2.3.1.75 genes atfA1 and atfA2, expression in Escherichia coli strain BL21(DE3) 674345 2.3.1.75 genes with high homologies to the Acinetobacter sp. ADP1 atfA are identified in the genome databases of several Gram-negative strains, including marine bacteria like Hahella chejuensis, psychrophilic strains like Psychrobacter sp., Polaromonas sp. and Bradyrhizobium japonicum. Ten atfA homologous genes are identified in the genome database of Arabidopsis thaliana 672451 2.3.1.75 isozyme AWAT1, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis 703312 2.3.1.75 isozyme AWAT2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis 703312 2.3.1.75 isozymes AWAT1 and AWAT2, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis 703312 2.3.1.75 MFAT, DNA and amino acid sequence determination and analysis, localized on the X chromosome, expression of the N-terminally FLAG-tagged enzyme in Spodoptera frugierda Sf9 cells using the baculovirus transfection system, or in COS-7 cells 659670 2.3.1.75 screening, DNA and amino acid sequence determination and analysis, expression in Escherichia coli strain JM109 674328